autoclaved micropipettes (Bio-Rad)
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Bio-Rad
autoclaved micropipettes
Autoclaved Micropipettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 869 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/autoclaved+micropipettes/Gene+Pulser+%2FMicroPulser+Electroporation+Cuvettes/pm36813480-36-140-152
Average 96 stars, based on 869 article reviews
Autoclaved Micropipettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 869 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/autoclaved+micropipettes/Gene+Pulser+%2FMicroPulser+Electroporation+Cuvettes/pm36813480-36-140-152
Average 96 stars, based on 869 article reviews
autoclaved micropipettes - by Bioz Stars,
2026-09
96/100 stars
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Polymerase Chain Reaction:Article Title: Recombineering 101: Making an in-Frame Deletion Mutant. Article Snippet: QIAquick PCR Purification Kit (QIAGEN 28106) Quick load 100-bp (New England Biolabs N0467) and 1-kb ladders (New England Biolabs N0468) SYBR Safe DNA Gel Stain (Invitrogen S33102) TAE buffer (10×; Invitrogen 15558-026) Taq DNA Polymerase 5000 U/mL, with ThermoPol buffer (New England Biolabs M0267) Equipment Centrifuge (refrigerated) with rotor that accommodates 50-mL tubes (e.g., Beckman Coulter Avanti, J series, with JA25.50 rotor) Centrifuge bottles, polycarbonate with caps, 50-mL (Beckman Coulter 357002) Culture tubes, glass ( 15-mm×150-mm, diameter × length) with plastic caps, sterile Electrophoresis gel casting and running apparatus Electrophoresis power supply Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot107856 629 Erlenmeyer flask (250-mL), sterile Gel apparatus (tray, comb, and gel box) Gel imager Glass spreader (made from a glass rod) or glass plating beads Incubator(s) (at 30 ̊C and 37 ̊C) Microcentrifuge tubes, 1.5-mL (Eppendorf 022363743) and 2.0-mL (Eppendorf 022363344), autoclaved Micropipettes (P2, P10, P20, P200, P1000) MicroPulser electroporation cuvettes, 2-mm Purification:Article Title: Recombineering 101: Making an in-Frame Deletion Mutant. Article Snippet: QIAquick PCR Purification Kit (QIAGEN 28106) Quick load 100-bp (New England Biolabs N0467) and 1-kb ladders (New England Biolabs N0468) SYBR Safe DNA Gel Stain (Invitrogen S33102) TAE buffer (10×; Invitrogen 15558-026) Taq DNA Polymerase 5000 U/mL, with ThermoPol buffer (New England Biolabs M0267) Equipment Centrifuge (refrigerated) with rotor that accommodates 50-mL tubes (e.g., Beckman Coulter Avanti, J series, with JA25.50 rotor) Centrifuge bottles, polycarbonate with caps, 50-mL (Beckman Coulter 357002) Culture tubes, glass ( 15-mm×150-mm, diameter × length) with plastic caps, sterile Electrophoresis gel casting and running apparatus Electrophoresis power supply Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot107856 629 Erlenmeyer flask (250-mL), sterile Gel apparatus (tray, comb, and gel box) Gel imager Glass spreader (made from a glass rod) or glass plating beads Incubator(s) (at 30 ̊C and 37 ̊C) Microcentrifuge tubes, 1.5-mL (Eppendorf 022363743) and 2.0-mL (Eppendorf 022363344), autoclaved Micropipettes (P2, P10, P20, P200, P1000) MicroPulser electroporation cuvettes, 2-mm Staining:Article Title: Recombineering 101: Making an in-Frame Deletion Mutant. Article Snippet: QIAquick PCR Purification Kit (QIAGEN 28106) Quick load 100-bp (New England Biolabs N0467) and 1-kb ladders (New England Biolabs N0468) SYBR Safe DNA Gel Stain (Invitrogen S33102) TAE buffer (10×; Invitrogen 15558-026) Taq DNA Polymerase 5000 U/mL, with ThermoPol buffer (New England Biolabs M0267) Equipment Centrifuge (refrigerated) with rotor that accommodates 50-mL tubes (e.g., Beckman Coulter Avanti, J series, with JA25.50 rotor) Centrifuge bottles, polycarbonate with caps, 50-mL (Beckman Coulter 357002) Culture tubes, glass ( 15-mm×150-mm, diameter × length) with plastic caps, sterile Electrophoresis gel casting and running apparatus Electrophoresis power supply Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot107856 629 Erlenmeyer flask (250-mL), sterile Gel apparatus (tray, comb, and gel box) Gel imager Glass spreader (made from a glass rod) or glass plating beads Incubator(s) (at 30 ̊C and 37 ̊C) Microcentrifuge tubes, 1.5-mL (Eppendorf 022363743) and 2.0-mL (Eppendorf 022363344), autoclaved Micropipettes (P2, P10, P20, P200, P1000) MicroPulser electroporation cuvettes, 2-mm Sterility:Article Title: Recombineering 101: Making an in-Frame Deletion Mutant. Article Snippet: QIAquick PCR Purification Kit (QIAGEN 28106) Quick load 100-bp (New England Biolabs N0467) and 1-kb ladders (New England Biolabs N0468) SYBR Safe DNA Gel Stain (Invitrogen S33102) TAE buffer (10×; Invitrogen 15558-026) Taq DNA Polymerase 5000 U/mL, with ThermoPol buffer (New England Biolabs M0267) Equipment Centrifuge (refrigerated) with rotor that accommodates 50-mL tubes (e.g., Beckman Coulter Avanti, J series, with JA25.50 rotor) Centrifuge bottles, polycarbonate with caps, 50-mL (Beckman Coulter 357002) Culture tubes, glass ( 15-mm×150-mm, diameter × length) with plastic caps, sterile Electrophoresis gel casting and running apparatus Electrophoresis power supply Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot107856 629 Erlenmeyer flask (250-mL), sterile Gel apparatus (tray, comb, and gel box) Gel imager Glass spreader (made from a glass rod) or glass plating beads Incubator(s) (at 30 ̊C and 37 ̊C) Microcentrifuge tubes, 1.5-mL (Eppendorf 022363743) and 2.0-mL (Eppendorf 022363344), autoclaved Micropipettes (P2, P10, P20, P200, P1000) MicroPulser electroporation cuvettes, 2-mm Electrophoresis:Article Title: Recombineering 101: Making an in-Frame Deletion Mutant. Article Snippet: QIAquick PCR Purification Kit (QIAGEN 28106) Quick load 100-bp (New England Biolabs N0467) and 1-kb ladders (New England Biolabs N0468) SYBR Safe DNA Gel Stain (Invitrogen S33102) TAE buffer (10×; Invitrogen 15558-026) Taq DNA Polymerase 5000 U/mL, with ThermoPol buffer (New England Biolabs M0267) Equipment Centrifuge (refrigerated) with rotor that accommodates 50-mL tubes (e.g., Beckman Coulter Avanti, J series, with JA25.50 rotor) Centrifuge bottles, polycarbonate with caps, 50-mL (Beckman Coulter 357002) Culture tubes, glass ( 15-mm×150-mm, diameter × length) with plastic caps, sterile Electrophoresis gel casting and running apparatus Electrophoresis power supply Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot107856 629 Erlenmeyer flask (250-mL), sterile Gel apparatus (tray, comb, and gel box) Gel imager Glass spreader (made from a glass rod) or glass plating beads Incubator(s) (at 30 ̊C and 37 ̊C) Microcentrifuge tubes, 1.5-mL (Eppendorf 022363743) and 2.0-mL (Eppendorf 022363344), autoclaved Micropipettes (P2, P10, P20, P200, P1000) MicroPulser electroporation cuvettes, 2-mm Electroporation:Article Title: Recombineering 101: Making an in-Frame Deletion Mutant. Article Snippet: QIAquick PCR Purification Kit (QIAGEN 28106) Quick load 100-bp (New England Biolabs N0467) and 1-kb ladders (New England Biolabs N0468) SYBR Safe DNA Gel Stain (Invitrogen S33102) TAE buffer (10×; Invitrogen 15558-026) Taq DNA Polymerase 5000 U/mL, with ThermoPol buffer (New England Biolabs M0267) Equipment Centrifuge (refrigerated) with rotor that accommodates 50-mL tubes (e.g., Beckman Coulter Avanti, J series, with JA25.50 rotor) Centrifuge bottles, polycarbonate with caps, 50-mL (Beckman Coulter 357002) Culture tubes, glass ( 15-mm×150-mm, diameter × length) with plastic caps, sterile Electrophoresis gel casting and running apparatus Electrophoresis power supply Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot107856 629 Erlenmeyer flask (250-mL), sterile Gel apparatus (tray, comb, and gel box) Gel imager Glass spreader (made from a glass rod) or glass plating beads Incubator(s) (at 30 ̊C and 37 ̊C) Microcentrifuge tubes, 1.5-mL (Eppendorf 022363743) and 2.0-mL (Eppendorf 022363344), autoclaved Micropipettes (P2, P10, P20, P200, P1000) MicroPulser electroporation cuvettes, 2-mm Spectrophotometry:Article Title: Recombineering 101: Making an in-Frame Deletion Mutant. Article Snippet: QIAquick PCR Purification Kit (QIAGEN 28106) Quick load 100-bp (New England Biolabs N0467) and 1-kb ladders (New England Biolabs N0468) SYBR Safe DNA Gel Stain (Invitrogen S33102) TAE buffer (10×; Invitrogen 15558-026) Taq DNA Polymerase 5000 U/mL, with ThermoPol buffer (New England Biolabs M0267) Equipment Centrifuge (refrigerated) with rotor that accommodates 50-mL tubes (e.g., Beckman Coulter Avanti, J series, with JA25.50 rotor) Centrifuge bottles, polycarbonate with caps, 50-mL (Beckman Coulter 357002) Culture tubes, glass ( 15-mm×150-mm, diameter × length) with plastic caps, sterile Electrophoresis gel casting and running apparatus Electrophoresis power supply Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot107856 629 Erlenmeyer flask (250-mL), sterile Gel apparatus (tray, comb, and gel box) Gel imager Glass spreader (made from a glass rod) or glass plating beads Incubator(s) (at 30 ̊C and 37 ̊C) Microcentrifuge tubes, 1.5-mL (Eppendorf 022363743) and 2.0-mL (Eppendorf 022363344), autoclaved Micropipettes (P2, P10, P20, P200, P1000) MicroPulser electroporation cuvettes, 2-mm |